Surface Defect and Wettability Engineering of Porous SnOx for Reliable Bioassays with High Selectivity and Wide Linear Dynamic Range
Yifan Zhou1，2, Zhenyao Ding1, Qinghao Sun1, Liping Chen1（陈礼平）*, Dandan Wang1, Xiaoguang Bao1,2, and Xinjian Feng1,2,3（封心建）*
1College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China
2Innovation Center for Chemical Science, Soochow University, Suzhou 215123, China
3Suzhou Institute for Advanced Research, University of Science and Technology of China, Suzhou 215123, China
J.Am. Chem. Soc. 2024, 146, 24, 16581-16589
Abstract: Bioassay systems that can selectively detect biomarkers at both high and low levels are of great importance for clinical diagnosis. In this work, we report an enzyme electrode with an oxygen reduction reaction (ORR)-tolerant H2O2 reduction property and an air–liquid–solid triphase interface microenvironment by regulating the surface defects and wettability of nanoporous tin oxide (SnOx). The enzyme electrode allows the oxygen that is required for the oxidase catalytic reaction to be transported from the air phase to the reaction zone, which greatly enhances the enzymatic kinetics and increases the linear detection upper limit. Meanwhile, the ORR-tolerant H2O2 reduction property of SnOx catalysts achieved via oxygen vacancy engineering greatly reduces the interferent signals caused by oxygen and various easily oxidizable endogenous/exogenous species, which enables the selective detection of biomarkers at trace levels. The synergistic effect between these two novel qualities features a bioassay system with a wide dynamic linear range and high selectivity for the accurate detection of a wide range of biomarkers, such as glucose, lactic acid, uric acid, and galactose, offering the potential for reliable clinical diagnosis applications.
链接：//pubs.acs.org/doi/10.1021/jacs.4c03341
