博彩平台

Pei She^a, Yanxin Chu^a, Chunwei Liu^b, Xun Guo^b, Kang Zhao^a, Jianguo Li^a*（李建国）, Haijing Du^a, Xiang Zhang^a, Hong Wang^b, Anping Deng^a*（邓安平）

^aThe Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering & Materials Science, Soochow University, Renai Road 199, Suzhou 215123, China

^b OptoTrace (Suzhou) Technologies, Inc., STE 316, Building 4, No. 218, Xinghu Street, bioBAY, Suzhou Industrial Park, Suzhou 215123, China

Analytica Chimica Acta ,Volume 906, 4 February 2016, Pages 139–147

An immunochromatographic test (ICT) strip was developed for ultrasensitive competitive immunoassay of Hg²⁺. This strategy was achieved by combining the easy-operation and rapidity of ICT with the high sensitivity of surface-enhanced Raman scattering (SERS). Monoclonal antibody (mAb) against Hg²⁺ and Raman active substance 4-mercaptobenzoic acid (MBA) dual labelled gold nanoparticles (GNPs) were prepared as an immunoprobe. The Raman scattering intensity of MBA on the test line of the ICT strip was measured for quantitative determination of Hg²⁺. The ICT was able to directly detect Hg²⁺ without complexing due to the specific recognition of the mAb with Hg²⁺. The IC₅₀ and limit of detection (LOD) of the assay for Hg²⁺ detection were 0.12 ng mL⁻¹ and 0.45 pg mL⁻¹, respectively. There was no cross-reactivity (CR) of the assay with other nineteen ions and the ICT strips could be kept for 5 weeks without loss of activity. The recoveries of the assay for water, human serum and urine samples spiked with Hg²⁺ were in range of 88.3–107.3% with the relative standard deviations (RSD) of 1.5–9.5% (n = 3). The proposed ICT was used for the detection of Hg²⁺ in urine samples collected from Occupational Disease Hospital and the results were confirmed by cold-vapor atomic fluorescence spectroscopy (CV-AFS). The assay exhibited high sensitivity, selectivity, stability, precision and accuracy, demonstrating a promising method for the detection of trace amount of Hg²⁺ in environmental water samples and biological serum and urine samples.