博彩平台

An enzyme-activatable probe with a self-immolative linker for rapid and sensitive alkaline phosphatase detection and cell imaging through a cascade reaction

Hongmei Zhang^a,   Chenglong Xu^a,   Jie Liu^a,   Xiaohong Li^a,   Lin Guo^*b(郭琳)  and   Xinming Li^*a(李新明)

^a College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou, 215123 China

^b Department of Biochemistry and Molecular Biology, Medical College of Soochow University, Suzhou, 215123 China

Chem. Commun. 2015, 51, 7031-7034

We report the design and synthesis of a novel probe (1) for ALP assay by incorporating a self-immolative linker between a phosphate moiety and resorufin. Because of its good biocompatibility and rapid cell internalization, this probe also exhibited great potential for real-time monitoring of endogenous phosphatase activity in living cells.

链接：

//pubs.rsc.org/en/Content/ArticleLanding/2015/CC/c5cc01005e#